Graph represents the mean value of three independent experiments ±s.e.m. Real-time RT-PCR on separated cells using EF1a as a reference gene. ( M) Relative expression of tnfa in eGFP- and GFP + cells in amputated larvae. Green gates represent eGFP + population and mean percentage of eGFP + population ±s.e.m is indicated. Tg(tnfa:eGFP-F) larvae were either kept intact (control) or amputated at 3 dpf, and cells were collected at 6 hr post-treatment. ![]() ( L) Graphed data of representative fluorescence-activated flow cytometry analysis of eGFP + cells in upon amputation. Dotted lines delimit the caudal fin, arrowheads show overlapping signals, and arrows show the pigments. ( K) tnfa mRNA and eGFP-F expressions were analyzed using microscopy at 6 hpA in amputated fins from 3 dpf Tg(tnfa:eGFP-F) larvae. coli-infected Tg(tnfa:eGFP-F) larvae, scale bar = 20 μm. ( J) Multi-scan confocal analysis of GFP expression in E. Arrows show auto-fluorescent xanthophores. ( F, G) eGFP fluorescence (green) was analyzed by fluorescent microscopy in ( F) intact (control) and ( G) amputated Tg(tnfa:eGFP-F) fins at 6 hpA, dotted lines outline the caudal fin, scale bar = 100 μm and at 16 hpi in Tg(tnfa:eGFP-F) larvae injected with ( H) PBS or ( I, J) E. ( E) Imaging of tnfa mRNA expression in the muscle at higher magnification, asterisks show muscle fibres, scale bar in ( B) = 100 μm and in ( E) = 50 μm. You can establish workflows for routine inspections with overlays that are superimposed onto your live image.( A– E) Tumour necrosis factor alpha ( tnfa) mRNA expression (blue, arrowhead) was detected by in situ hybridization using tnfa anti-sense probe: at 6 hpA in ( A) intact (control) and ( B) amputated fins from 3 dpf WT larvae, ( C) in uninfected larvae (54 hpf, hours post-fertilization) and ( D, E) E. ![]() Working with overlays provides multiple advantages, especially for recurring tasks, for example checking if parts or components comply to specific parameters. The monitor can be quickly and easily adjusted for multiple users. The Leica DMS1000 is inherently ergonomic! This is due to the completely digital output of the digital microscope system as there are no eyepieces to adjust for each user. The built-in CMOS camera can capture 5 Mpixel still images or Full-HD movie clips within seconds directly to a SD card without the need of a computer. Functions such as capture / review images or movie clips can be selected by a simple push of the button. The Leica DMS1000 settings are customizable via an IR wireless remote control. This nearly eliminates any image delays because of the fast camera, making inspection of the product/biological sample, or observation of an experiment, simple and easy. Fast Live Imagesįast, high resolution live images in Full HD resolution with up to 30fps. Industry Applicationsĭigital Microscope System Leica DMS1000 for Industry Applications Life Science Applicationsĭigital Microscope System Leica DMS1000 for Life Science Applications Built-in Encoded ZoomĢD-Measurement without a computer! The coded zoom feature of the Leica DMS1000 allows the user to make quick measurement checks using a scale bar, that automatically adjusts for each zoom setting. The Leica DMS1000 with coded zoom optics can be used as stand-alone or connected to a computer offering accurate 2D-measurements by using Leica Application Suite (LAS) software.įor special diagnostics requirements, there is the Leica DMS1000 B, a version certified for in-vitro-applications like in-vitro-fertilization (IVF). High-quality, full color still images as well as Full-HD movies are possible. Take a fresh look at your samples on a monitor without the need of eyepieces. – Leica LAS software for image acquisition on a computer – Ergonomic work thanks to highly convenient operation – Digital camera with 5.0-megapixel resolution – FlexAperture™ technology provides for constant brightness of the image across the entire zoom range – Parfocal optics – no refocusing when changing the magnification – Digital magnification range of up to 300X – 8:1 zoom range (with switchable clickstops) ![]() ![]() The built-in HDMI microscope camera provides full high definition live images of up to 30fps and a resolution of 5Mpixels. From tiniest detail to an overview, the optics ensures magnification of up to 300x. Digital microscope system for digital inspection, observation and measurement.
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